Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells

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Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells

Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular st...

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Nosema bombycis (Microsporidia) suppresses apoptosis in BmN cells (Bombyx mori).

Nosema bombycis (N. bombycis, Nb) is a fungus-related and obligate intracellular parasite that causes chronic pebrine disease in the silkworm. After infecting the host, spores obtain energy from host cells and survive for several days. This symbiosis between the pathogen and the host cell suggests that N. bombycis prevents apoptosis and reactive oxygen species (ROS) production of host cells to ...

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synthesis of platinum nanostructures in two phase system

چکیده پلاتین، فلزی نجیب، پایدار و گران قیمت با خاصیت کاتالیزوری زیاد است که کاربرد های صنعتی فراوانی دارد. کمپلکس های پلاتین(ii) به عنوان دارو های ضد سرطان شناخته شدند و در شیمی درمانی بیماران سرطانی کاربرد دارند. خاصیت کاتالیزوری و عملکرد گزینشی پلاتین مستقیماً به اندازه و- شکل ماده ی پلاتینی بستگی دارد. بعضی از نانو ذرات فلزی در سطح مشترک مایع- مایع سنتز شده اند، اما نانو ساختار های پلاتین ب...

A comparative study on artificial germination of two microsporidia under the neutralization method

Lamerin microspridia (Lbms) isolated from Lamerin breed of the silkworm, Bombyx mori, L. and the standard strain Nosema bombycis, were induced to germinate artificially by two-step procedure. Fresh percoll purified spores were activated by incubating them in potassium hydroxide solution (KOH) (pH 11.00) and germinated artificially by using phosphate buffer saline (PBS), (pH 7.00). Germinated sp...

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Functional characterization of an aquaporin from a microsporidium, Nosema bombycis

Microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. During the germination process, there is an increase in the osmotic pressure of microsporidian spores. As part of this study, we cloned a homologous aquaporin gene in Nosema bombycis, and named it Nosema bombycis aquaporin (NbAQP). Sequence analysis revealed ...

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ژورنال

عنوان ژورنال: PLOS ONE

سال: 2017

ISSN: 1932-6203

DOI: 10.1371/journal.pone.0179618